O8264
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 | Immunofluorescence Human Jurkat cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 5 μg/mL Anti-Orai1 (Cat. No. O8264). The antibody was developed with Goat Anti-Rabbit IgG, Cy3 conjugate. |
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O8264
Sigma
Anti-ORAI1 antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
| Synonym: | Anti-Calcium release-activated calcium channel protein 1, Anti-CRACM1, Anti-TMEM142A, Anti-Transmembrane protein 142A |
Description
| Immunogen | synthetic peptide corresponding to amino acids 288-301 of human ORAI1, conjugated to KLH. The corresponding sequence differs by 2 amino acids in rat and mouse. |
| Physical form | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
Properties
| antibody form | affinity isolated antibody |
| form | buffered aqueous solution |
| mol wt | antigen mol wt ~35 kDa (non-glycosylated) |
| antigen mol wt ~50 kDa (glycosylated) | |
| species reactivity | human |
| availability | not available in Canada (due to temporary import restrictions; for questions or status updates, please email us at antibody.canada@sial.com) |
| concentration | ~1.0 mg/mL |
| application(s) | immunoblotting: 1-2 μg/mL using whole extract of human HEK-293T cells expressing ORAI1 |
| indirect immunofluorescence: 10-20 μg/mL using Jurkat cells | |
| Gene Information | human ... ORAI1(84876) |
| mouse ... Orai1(109305) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |
